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How to deal with mushroom bag contamination
During the indoor cultivation process, the bags should be turned 4 to 5 times. The first turn should occur after 6 to 7 days of inoculation, and then every 7 to 10 days thereafter. It’s important to regularly inspect the bags for signs of contamination and address any issues promptly to prevent spread.
If mild contamination is detected—such as small spots or filamentous microorganisms at the seams or folds of the bag—it can be treated. Use a syringe to draw 50 ml of 75% alcohol mixed with 30 ml of 36% formaldehyde. Inject this solution into the affected area and gently massage the surface to ensure the liquid reaches the contaminated spot. Then, cover the injection site with tape to seal it and prevent further contamination.
In cases where the contamination occurs at the inoculation point, but the mycelium is still actively growing and not yet affected, you can apply a 5–10% lime water supernatant followed by a 50% carbendazim solution. However, avoid applying the solution directly onto the shiitake mushroom mycelium to prevent damage.
If dead or heavily contaminated areas are found, they should be removed and the bag re-inoculated under sterile conditions. For bags that are severely contaminated—covered in visible bacterial spots—the best approach is to discard the bag, break it open, and mix the contents with a 3% lime solution. Let it sit overnight, then spread it out to dry before re-dosing, bagging, sterilizing, and re-inoculating.
Regular monitoring and quick action are essential to maintaining a healthy culture and preventing the spread of contaminants throughout the entire batch. Always maintain good hygiene and follow proper sterilization protocols to ensure the success of your cultivation process.