Stone frog artificial propagation technology

The stone frog, also known as broiler, is a species of amphibians, anarches, and frogs. It is one of the largest edible frogs in China, and its nutritional value is comparable to that of soft-shelled turtles.
1 species of frog selection of natural species of frog spawning season in May-August, the end of May to early June for the spawning season. Spawning is generally performed twice a day, with 1,000 to 2,000 eggs spawned each time. The second spawning quantity is less than the first. The conditions for selecting a species of frog are: damage to the frog body and strong activity. Female frogs weigh more than 200 grams and male frogs weigh more than 150 grams. The frogs purchased on the day should be washed with clear water, or washed with potassium permanganate solution at a concentration of 10-15 mg/kg, and then replaced with clear plastic bottles.
2. Equipment and Equipment
(1) The nursery houses are built with brick concrete walls, 1.5 meters high, 3 meters high, and covered with white plastic tiles or solar panels to maintain sufficient light. The ground must be cemented to facilitate water flow. operating. The area of ​​0.5 square meters can produce about 10,000 oysters. The size of the building depends on the size.
(2) Nursery frame wooden structure, height 70 cm, width 40 cm, long depending on the size of the seedlings.
(3) Seedling tray white hard plastic plate with specifications of 39.557.5l1.5 (cm).
(4) Self-made wood frame of hatching screen, a nylon screen cloth (80-100 mesh per square centimeter) is pulled inside. Frame size 31.551.53 (cm).
(5) Automatic temperature control electric heating rods temperature control range of 16-32 °C, 220 volts, one tray per seedling.
(6) Fertilizer enamel saucer can be about 8 cm in diameter.
(7) syringe 1 ml syringe with 5 needle, 20 ml syringe with 7.5 needle.
(8) The tweezers require a thin, elastic stainless steel tweezers, with a length of no more than 15 cm.
(9) Precipitation and water storage dual-purpose pool brick cement structure, the water-soluble amount is 1-2 tons.
Separate thermometers, straws, stain-removing cloths, semen cloths, hormones, medicines, etc.
3. Operating procedures
(1) Artificial insemination Before mature artificial insemination, mature males must be prepared. More than 150 grams of body weight will scream. According to the number of female frogs 2:1 or 3:1. The female frog body is more than 200 grams, and when it is found that the pro-fauna estrus enters the oviposition time (after 10 hours after the injection of oxytocic acid), the examination should be immediately carried out. If there is ooze out of the abdomen of the lightly pressed female frog, the egg should be immediately squeezed into the fertilization dish. Immediately squeeze the semen into the fertilization dish in the same way and mix gently with the feather for about 1-2 minutes to fully mix the eggs. While slowly adding clean water, and gently stirring for about 1 minute or so, dump the sewage. After washing the eggs with water, the fertilized eggs are transferred to the hatchery and incubated.
(2) artificial hatching first, row operation. The fertilized eggs will be spread gently on the hatching screen with tweezers, adding fresh water, so that the eggs just floated on the water surface. Second, water temperature regulation. The temperature in the insemination dish should be consistent with the temperature in the hatchery tray. Third, change the water. During the incubation process, change the water 1-2 times. When the water is changed, the temperature difference must not exceed 2°C. Eggs that have not been fertilized or have been fertilized and are killed by certain influencing factors must be carefully removed. Otherwise, water quality will deteriorate. Affects embryonic development. The length of incubation is related to water temperature and the amount of oxygen in the water. It usually takes 3-5 days.

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