Ultrasonic extraction of flavonoids from common Chinese herbal medicines

Flavonoid ultrasonic extraction

The efficacy of flavonoids is multi-faceted. It is a strong antioxidant, which can effectively eliminate oxygen free radicals in the body, anti-oxidation and anti-aging. Flavonoids can improve blood circulation, lower cholesterol, and flavonoids can inhibit inflammatory enzymes. Exudation can improve wound healing and pain relief. Because of its strong anti-histamine, it can be used in all kinds of sensitive diseases. Therefore, it has been sought after by many scientists, and they are all studying how to extract more flavonoids from plants. The literature of Ronghui Ultrasound lists the ultrasonic extraction methods of flavonoids in various plants one by one for easy reference. Remarks: The following methods are all from the identifiable literature and are for reference only.

1. Ultrasonic extraction of flavonoids from Ginkgo biloba. Experiments show that the ultrasonic extraction method is superior to the Soxhlet extraction method. The optimal operation bar of the ultrasonic extraction method is as follows: ultrasonic frequency 40KHZ, processing time 10min, and standing time 12h.

2. Ultrasound-assisted extraction of flavonoids from pomelo peel. Experiments show that the optimal extraction conditions for pomelo flavonoids are: ultrasonic power 1250W, ultrasonic time 10min, ratio of material to liquid 1:30 (g/ml).

3. Ultrasonic extraction of flavonoids from Eucommia ulmoides leaves. The experiment showed that: Eucommia ulmoides leaves as raw material, 40% ethanol as extract, m (eudox leaves): m (extract) = 1:60, soaked for 2.5 h, each time ultrasonic for 1 h, repeated treatment twice. Under this condition, the flavonoid extraction rate was 17.14%.

4. Ultrasonic extraction of total flavonoids from breviscapus. The experiment showed that: the method of ultrasonic extraction, reflux extraction and water extraction and alcohol precipitation method were used to extract the total flavonoids from Erigeron breviscapus, and the extraction rate and extraction time of the three methods were compared. RESULTS: The ethanol extraction and extraction rates of ultrasonic extraction and reflux extraction were equal, higher than the water extraction and alcohol precipitation method, but the extraction time of ultrasonic extraction method was 40 min, which greatly shortened the extraction time compared with other methods. Conclusion: Ultrasonic technology is effective in extracting total flavonoids from Erigeron

5. Ultrasonic extraction of total flavonoids from Epimedium. The experiment showed that the best extraction process of the active ingredient of Epimedium was 80% ethanol, the ultrasonic frequency was 40 kHz, and the extraction time was 30 min. The extraction temperature was room temperature.

6. Ultrasonic extraction of flavonoids from licorice. Experiments show that ultrasonic extraction at room temperature for 80 min is more suitable. At this time, the content of flavonoids was 1.311%, the average recovery was 99.10%, and the RSD was 0.990% (n=6). Conclusion: The extraction of licorice flavonoids by ultrasonic technology is faster and the experimental results are satisfactory.

7. Ultrasonic extraction of peony flavonoids. The experiment shows that the best process of ultrasonic extraction is that 70% ethanol, the ratio of material to liquid is 1:20, and the ultrasonic extraction rate is 22 kHz for 10 min, the extraction rate can reach 91.5%. It has the advantages of high extraction efficiency, short extraction time and low temperature.

8. Optimization of ultrasonic assisted extraction process of total flavonoids from Cyclocarya paliurus L. The experiment showed that the extraction solvent was 60% ethanol, the liquid-to-liquid ratio was 55 mL/g, the ultrasonic treatment time was 34 min, and the ultrasonic power was 720 W. Under the optimal conditions, the yield of total flavonoids was 86.63%, and the optimization process was stable. The rate is also high, and the test verification results are consistent with the model prediction values.

9. Ultrasonic extraction of total flavonoids from Tamarix chinensis. The experiment showed that the optimum extraction conditions of total flavonoids from Tamarix chinensis were 1:15, the sonication time was 40 min, the ethanol concentration was 45%, and the ultrasonic temperature was 50 °C. According to the experiment, the average yield of total flavonoids of Tamarix chinensis was 33.675 mg/g, and the content was 22.75%. Conclusion: The experimental results are reliable, simple, time-saving and non-polluting. It is an effective way to extract flavonoids from Tamarix chinensis.

10. Ultrasonic extraction of lotus leaf flavonoids. The experimental results show that the optimal extraction conditions are ethanol concentration 50%, solid-liquid ratio 1:20, extraction 3 times, ultrasonic extraction time 25 min. Under the optimal combination of parameters, the extraction rate of flavonoids in lotus leaves is 4.85%.

11. Study on ultrasonic extraction technology of edible cactus flavonoids. The experiment showed that the cactus dry powder was used as the raw material, and the volume fraction of 73.6% ethanol was used as the solvent, and the ratio of material to liquid was 1:10 (m:V). The extraction time was 18.8 min.

12. Ultrasonic extraction of flavonoids from hawthorn leaves. Studies have shown that the optimum extraction process of hawthorn leaf flavonoids is temperature 80 ° C ~ 85 ° C, liquid to material ratio (ethanol volume: raw material quality) 18:1, ultrasonic extraction with 60% ethanol 2 times, each 40min.

13. The best technology for the extraction of flavonoids from Astragalus by ultrasonic method. The experiment showed that the medicinal material was pulverized into coarse powder, and 15 times of 80% ethanol was added. After extraction at 70 °C for 30 min, the extraction was continued twice, and the total extraction rate of flavonoids was 98.27%. Conclusion This process is simple and feasible, and has good reproducibility and is suitable for industrial production.

14. Ultrasonic extraction of flavonoids from Platycladus orientalis leaves. The experiment showed that the optimal conditions for the extraction of flavonoids from the leaves of Platycladus orientalis by ultrasonic method: 50% ethanol solution, temperature 70 ° C, 40 kHz sonication for 40 min. Under these conditions, the extract was evaporated to dryness to obtain crude extract. The extraction rate reached 93.60%, which was 23.4% higher than that without ultrasonic treatment. The results of qualitative test and UV spectroscopy indicated that the flavonoids in the leaves of Platycladus orientalis were mainly flavonols. Compared with traditional alcohol extraction, ultrasonic method It has obvious advantages such as stability, energy saving and high extraction rate.

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