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In the breeding seedlings, commonly used unicellular algae are Chaetoceros, Nitzschia closterium, Phaeodactylum tricornutum, Chrysophyta, Planophyta, Chlorella, Micrococcus sp.
First, Chaetoceros, Nitzschia closterium, Phaeodactylum tricornutum, Nitzschia closterium, Phaeodactylum tricornutum are diatoms. There are many kinds of Chaetoceros, commonly used mainly Chaetoceros muelleri. Chaetoceros mutans is a broad-spectrum and broad-salinity algae that can be cultivated in both southern and northern China. It grows fastest when the water temperature is between 28°C and 30°C and salinity is about 30°C. As its culture solution can be, if the amount of additional silicon, the growth rate will be significantly improved. The light intensity should be adjusted according to different levels of cultivation and different concentrations of algae, such as in the first and second culture should avoid direct light, light can not be too strong, three-level culture just when inoculated with weak light, better when the concentration It can be irradiated with direct light and at the same time, the illumination time can be appropriately increased. Generally, it can be extended by 2 to 3 hours. In the first and second cultures, when the light is too strong for a long time or the temperature exceeds 30°C, the algae species tend to turn green. At this time, the light intensity, light time, and temperature should be strictly controlled, and the salinity of the culture liquid can be reduced when necessary. To make it back to normal. The temperature for the growth of Nitzschia closterium and Phaeodactylum tricornutum is 15 °C ~ 20 °C, and it will die if it exceeds 28 °C. However, I used to grow them at 31°C in combination with Chlorella 3011, and the concentration reached 600,000/mL.
Second, the Jin algae culture The commonly used golden algae shellfish Jin algae 3011, Zhanjiang fork algae, and other whipping algae, its wide temperature, the optimum temperature of 28 °C ~ 30 °C, the optimal salinity of 25 ‰ ~ 32 inches. The amount of vitamins and iron should be added during the culture of golden algae to increase its growth rate. In the tertiary culture, the protozoa breed very fast. When two or more protozoa per field are observed under a microscope at 100 times, the algae It can become clear water within 2 days. Therefore, we should strictly disinfect water, tools, and culture ponds, and conduct microscopic examination of algae species every day to timely feed the algae with a small amount of pollution. The algae that cannot be used for serious pollution should be eliminated first Drop to prevent contamination of other pools.
Third, Chlorella and Microchicoccus Chlorella, Micrococcus is Chlorophyta, Chlorophyta, is a wide temperature, salty algae. Its own anti-pollution characteristics, easy to culture, in the three-stage culture, the water can be used without disinfection, can be filtered with a 300-mesh sieve, but should be regularly added to the culture medium to prevent aging, the serious pollution of chlorella and The microalgae can be used to disinfect algae species with a certain concentration of bleaching agents to kill their protozoa. After the protozoa are killed, they can be reduced with sodium thiosulfate and return to normal after two days. In recent years, due to the massive rise of marine aquaculture, the quality of seawater has changed greatly. In particular, the increase of diatom content in seawater has brought great difficulties to the cultivation of green algae. In the process of culturing chlorella and micrococcus in Hainan Provincial Fisheries Research Institute, the author encountered a few times when the concentration of algae was high, the algae quickly turned red within 1 hour, even if strict disinfection of water was not effective. This phenomenon usually occurs in July to September each year. At this time, adding a certain amount of Bacillus can restore normal.
4. The genus Chlorella is a genus Chlorophyta, a phytoplankton genus, and genus Platymonas, which is also one of the most easily cultivated algae species. This algae species also has a large absorption of iron and should be supplemented with iron to accelerate its growth. . At a higher concentration of Platymonas, it can also be purified, concentrated, and cryopreserved by adding a certain concentration of the drug, and when necessary, the culture medium can be added to culture again.
Fifth, in the shellfish algae cultivation should also pay attention to the following points:
1. Strictly sterilize water, fertilizer, tools, and culture pools.
2. Add appropriate amount of nutrient salts according to the fat of the sea in each sea area. Adding low-concentration nutrient salts can shorten the stagnation period of algae cells, and additional nutrients can maintain its exponential growth phase later.
3, timely adjustment of light, salinity, temperature. According to the different concentrations of algae in time to adjust the light intensity, light time, when the concentration is greater, the light intensity can be enhanced, prolong the light time, algae species prone to variation in algae, in the first-level culture to adjust the growth of salinity and temperature is very necessary.
4, according to different types of algae with different drugs to control pollution and when the concentration of algae is high, timely concentration, preservation in preparation for the occasion.
5. In the first-stage culture, macroscopic purification should be carried out according to the floatation and concentration characteristics of the algae at each time of seed expansion to ensure the purity and activity of the algae species.
Mycelium culture technology of shellfish
In recent years, as a bait, monomonas have played an increasingly important role in shellfish breeding. The quality of monocytic algae culture has become the key to the success of shellfish breeding. How to cultivate algae with high purity, high density, less bacteria and fresh growth directly relates to the quality and quantity of shellfish seedlings. In July 1995 to August 2004, the author was engaged in the cultivation of shellfish monocystophytes at the Haizheng Pilot Station of the Fisheries Administration of Weihai City and the Fisheries Research Institute of Hainan Province. The following is a discussion of practical results and personal experiences for technical personnel. Reference and communication.