1. Paraffin sections were placed in an oven at 67 ° C, baked for 2 hours, dewaxed to water, and rinsed three times with PBS pH 7.4 for 3 minutes (3 x 3'). 2. Take a certain amount of pH=6.0 citrate buffer solution, add it to the microwave box, heat it to boiling in the microwave, place the dewaxed hydrated tissue section on the high temperature resistant plastic slice rack, and put it into the boiling buffer. Mid-range microwave treatment for 10 minutes, take out the microwave box to drain the water naturally, remove the slide from the buffer, rinse twice with distilled water, then rinse 2×3' with PBS. (Note: not all antibodies require microwave repair, depending on the situation) 3. Add 1 drop of 3% H 2 O 2 per section and incubate for 10 minutes at room temperature to block endogenous peroxidase activity. Rinse 3 x 3' with PBS. 4. Remove the PBS solution and add 1 drop of the corresponding primary antibody (corresponding dilution factor) to each section and incubate for 2 hours at room temperature. 5, PBS rinse 3 × 5 '. The PBS solution was removed and 1 drop of polymer enhancer was added to each section and incubated for 20 minutes at room temperature. Rinse 3 x 3' with PBS. 6. Remove the PBS solution, add 1 drop of enzyme-labeled anti-mouse/rabbit polymer to each section, and incubate for 30 minutes at room temperature. Rinse 3 x 5' with PBS. 7. Remove the PBS solution and add 1 drop of freshly prepared DAB solution (diaminobenzidine) to each section and observe for 5 minutes under a microscope. 8, hematoxylin counterstaining, 0.1% HCl differentiation, tap water rinse, blue, sliced ​​by gradient alcohol dehydration, xylene transparent, neutral gum seal, dry and observe. Cruthes And Walking Aids,Arm Crutch,Disabled Crutch,Aluminum Crutch For Disabled Haloxylon Ammodendron Medical Equipment Co., Ltd. , https://www.haloxylonmedical.com
Immunohistochemistry technology topic: immunohistochemistry Elivison two-step method
Luoyang Haloxylon Ammodendron Medical Device Co., Ltd. was founded in 2019, is a professional engaged in medical devices, home medical devices and related health products, product development and sales as one. The factory strictly implements ISO 13485 standard, all products are in line with medical CE standard. At present, our business has a sound economic foundation and strong relationship network, many new traders in Europe, America, Australia and China have appointed us as their sole agent. Perfect technology and personnel allocation, in solving all kinds of solutions for customers at the same time, but also for customers to explore the Chinese market, find sources of goods, accept sources of goods, and for the enterprise carefully create professional, personalized, all-weather, all-round one-stop service. In mainland China, we have a very efficient import and export service system and we work closely with many shipping companies and airlines. For the export of your goods, we can provide you with the best shipping, air transportation, customs clearance, insurance and other services. At present, the company's trade scale is expanding day by day, customers all over the world, and with good reputation, won the trust of customers at home and abroad, to promote China's modernization, promote economic and technical exchanges with the world, to enhance the friendship with the people of all countries have done a lot of useful work. If you need more information, please feel free to contact us