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Natural seeding: This method is still used in some remote areas where there is no modern seed production equipment and technology.
1 old grass species: mushrooms after the first batch of mushrooms after harvest, the second batch of mushrooms to grow, choose to produce more mushrooms, mushroom growth consistent, mycelium exuberant, no pests and diseases, the straw out, in the ventilation After drying, deposit in a clean and dry tile tank and seal it with soil. In the second month of growing mushrooms, remove the grass seeds, sprinkle rice bran and water, and stack them for three days.
2 Cap Species: After selecting the big ones, after the parachute is opened, the mushroom pleats will turn red. Take the following: Cut the mushroom stalks, dry them in a ventilated place, wrap them in clean paper, and store them in the tank. In the second year, the mushroom is removed from the water and soaked in water for 1 to 2 hours. The water that has been soaked with mushrooms is used as a strain to pour on the straw of the mushroom and the new mycelia can be produced through the spores.
Pure seed preparation: Good strains can only be obtained by pure seed preparation.
1 parent species preparation: using spore separation or tissue separation. Select the seven to eight ripe ones that appear on the second day of the first batch of mushrooms and separate the mushrooms with good shape, good color, no pests, single or triple groups. The medium can be used: (1) potato glucose medium, (2) potato 200 g, sucrose 20 g, yeast extract 6 g, ammonium sulfate 3 g, agar 20 g, water 1000 ml, pH 7.2-7.4. After isolation, the culture is conducted at 30 to 35 degrees Celsius for 8 to 10 days. The mycelium grows from white to yellowish to reddish-brown sputum, indicating that the strains are mature.
2 Preparation of original species and cultivars: The original species is derived from the expansion of the parent species, and the original species can be expanded into cultivars and can also be used directly as cultivars. The culture medium of the original species and the cultivar is the same as that of the seed bottle, and the inoculation method is basically the same. The culture medium can be used: (1) 80% of rice straw is cut into 3 to 4 cm segments, rice bran or bran is 20%, and water is appropriate. Practices: chopped straw, soaked in water for 16 to 24 hours, after filtering the water to add rice bran, mix well, water content in the hands of the fingers to seep between the water is appropriate, sub-packed in 750 ml bacteria species bottle, daily 121 degrees Celsius sterilization for 30 minutes, continuous sterilization for two days. (2) 86% of wheat grain, 8.6% of chaff, 4.3% of cow dung powder, 0.4% of calcium carbonate, 0.2% of potassium dihydrogen phosphate, and 0.5% of lime. Practice: Refer to the method of making wheat seeds. One cane mother can be connected to 4 to 6 bottles of the original species, a bottle of original species can be connected to 40 to 50 bottles of cultivars. The parent species should control the transfer tube no more than five times to maintain hypha vitality. The original species should not increase the generation. Otherwise, the hyphae viability will be reduced and the strains will be degraded due to more generations. The original species and cultivars can be cultured at 30-32 degrees Celsius after inoculation, and the mycelium grows toward the bottom of the bottle. When it is nearly mature, the culture temperature is adjusted to 28-30 degrees Celsius, which is conducive to enhancing the ability to form fruit bodies. Mature bacteria can see the internal red-brown bacterial mass on the wall of the bottle.
How to make a good straw mushroom strain?
The excellent grass mushroom strains should be mycelium with strong viability, high yield, and good quality of mushrooms. Quality straw mushrooms should be thick and tough, not easy to open umbrellas, mushroom shape, shiny, good taste when eating, with flavor.
5A grade Huangjiu is adopted for Aged Wine,i.e. A grade raw materials, A grade timing, A grade pottery jar, A grade storage and A grade brewers. Using Huangjiu of the most representative years as base, aged wine is bottled in green porcelain for banquet and gift-giving. Glass-bottled-wine is mainly used for 3-5 years of ordinary wine, with superior cost performance, suitable for people who drink at home frequently.